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CAOL enhances AMPK activity and suppresses the TGF‐ β /Smad pathway in myocardial tissue. (A–D) The mRNA levels of AMPK , TGF-β , SMAD2 , and <t>SMAD3</t> were detected, respectively, by RT‐qPCR ( n = 6). (E–L) WB analysis and quantification of the protein levels of AMPK, p‐AMPK, TGF‐ β 1, Smad2, p‐Smad2, Smad3, and <t>p‐Smad3</t> ( n = 6). Data were shown as mean ± SD, ## p < 0.01 compared with the sham group. ∗ p < 0.05, ∗∗ p < 0.01 compared with the model group, ns means nonsignificant.
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CAOL enhances AMPK activity and suppresses the TGF‐ β /Smad pathway in myocardial tissue. (A–D) The mRNA levels of AMPK , TGF-β , SMAD2 , and <t>SMAD3</t> were detected, respectively, by RT‐qPCR ( n = 6). (E–L) WB analysis and quantification of the protein levels of AMPK, p‐AMPK, TGF‐ β 1, Smad2, p‐Smad2, Smad3, and p‐Smad3 ( n = 6). Data were shown as mean ± SD, ## p < 0.01 compared with the sham group. ∗ p < 0.05, ∗∗ p < 0.01 compared with the model group, ns means nonsignificant.
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CAOL enhances AMPK activity and suppresses the TGF‐ β /Smad pathway in myocardial tissue. (A–D) The mRNA levels of AMPK , TGF-β , SMAD2 , and <t>SMAD3</t> were detected, respectively, by RT‐qPCR ( n = 6). (E–L) WB analysis and quantification of the protein levels of AMPK, p‐AMPK, TGF‐ β 1, Smad2, p‐Smad2, Smad3, and p‐Smad3 ( n = 6). Data were shown as mean ± SD, ## p < 0.01 compared with the sham group. ∗ p < 0.05, ∗∗ p < 0.01 compared with the model group, ns means nonsignificant.
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CAOL enhances AMPK activity and suppresses the TGF‐ β /Smad pathway in myocardial tissue. (A–D) The mRNA levels of AMPK , TGF-β , SMAD2 , and SMAD3 were detected, respectively, by RT‐qPCR ( n = 6). (E–L) WB analysis and quantification of the protein levels of AMPK, p‐AMPK, TGF‐ β 1, Smad2, p‐Smad2, Smad3, and p‐Smad3 ( n = 6). Data were shown as mean ± SD, ## p < 0.01 compared with the sham group. ∗ p < 0.05, ∗∗ p < 0.01 compared with the model group, ns means nonsignificant.

Journal: BioMed Research International

Article Title: Compound Astragalus Oral Liquid Attenuates Ventricular Remodeling in Heart Failure After Myocardial Infarction via the AMPK/TGF‐ β /Smad Signaling Pathway

doi: 10.1155/bmri/1276968

Figure Lengend Snippet: CAOL enhances AMPK activity and suppresses the TGF‐ β /Smad pathway in myocardial tissue. (A–D) The mRNA levels of AMPK , TGF-β , SMAD2 , and SMAD3 were detected, respectively, by RT‐qPCR ( n = 6). (E–L) WB analysis and quantification of the protein levels of AMPK, p‐AMPK, TGF‐ β 1, Smad2, p‐Smad2, Smad3, and p‐Smad3 ( n = 6). Data were shown as mean ± SD, ## p < 0.01 compared with the sham group. ∗ p < 0.05, ∗∗ p < 0.01 compared with the model group, ns means nonsignificant.

Article Snippet: After blocking with nonfat milk at room temperature, membranes were incubated overnight at 4°C with primary antibodies against AMPK (CST, United States), p‐AMPK (CST, United States), TGF‐ β 1 (Proteintech, China), Smad2 (Proteintech, China), Smad3 (Proteintech, China), p‐Smad2 (CST, United States), p‐Smad3 (CST, United States), and GAPDH (Proteintech, China).

Techniques: Activity Assay, Tissue, Quantitative RT-PCR, Analysis

CAOL enhances AMPK activity and suppresses the TGF‐ β /Smad pathway in myocardial tissue. (A–D) The mRNA levels of AMPK , TGF-β , SMAD2 , and SMAD3 were detected, respectively, by RT‐qPCR ( n = 6). (E–L) WB analysis and quantification of the protein levels of AMPK, p‐AMPK, TGF‐ β 1, Smad2, p‐Smad2, Smad3, and p‐Smad3 ( n = 6). Data were shown as mean ± SD, ## p < 0.01 compared with the sham group. ∗ p < 0.05, ∗∗ p < 0.01 compared with the model group, ns means nonsignificant.

Journal: BioMed Research International

Article Title: Compound Astragalus Oral Liquid Attenuates Ventricular Remodeling in Heart Failure After Myocardial Infarction via the AMPK/TGF‐ β /Smad Signaling Pathway

doi: 10.1155/bmri/1276968

Figure Lengend Snippet: CAOL enhances AMPK activity and suppresses the TGF‐ β /Smad pathway in myocardial tissue. (A–D) The mRNA levels of AMPK , TGF-β , SMAD2 , and SMAD3 were detected, respectively, by RT‐qPCR ( n = 6). (E–L) WB analysis and quantification of the protein levels of AMPK, p‐AMPK, TGF‐ β 1, Smad2, p‐Smad2, Smad3, and p‐Smad3 ( n = 6). Data were shown as mean ± SD, ## p < 0.01 compared with the sham group. ∗ p < 0.05, ∗∗ p < 0.01 compared with the model group, ns means nonsignificant.

Article Snippet: After blocking with nonfat milk at room temperature, membranes were incubated overnight at 4°C with primary antibodies against AMPK (CST, United States), p‐AMPK (CST, United States), TGF‐ β 1 (Proteintech, China), Smad2 (Proteintech, China), Smad3 (Proteintech, China), p‐Smad2 (CST, United States), p‐Smad3 (CST, United States), and GAPDH (Proteintech, China).

Techniques: Activity Assay, Tissue, Quantitative RT-PCR, Analysis